Prevalence of Plasmid and Antimicrobial resistance in Pseudomonas aeruginosa Isolated from surgical wound infections in Nigeria

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J. I. Ehiaghe
P. O Nwiyi


The study was conducted to determine the antimicrobial resistance and plasmid DNA profile of Pseudomonas aeruginosa isolated from patients with clinically diagnosed post- operative surgical wound infections. The research was carried out in university of Benin teaching hospital (UBTH) and Nnamdi Azikiwe university teaching hospital (NAUTH). The work adopted a cross- sectional survey and the sample were obtained using the randomized sampling method. Samples were cultured on MacConkey and blood agar media and incubated anaerobically and aerobically for 24hours. Phenotypic characterization of bacterial isolates was confirmed biochemically. Antibiotic susceptibility testing was carried out by disc diffusion method on Mueller- Hinton agar. There were high prevalence (78% and 85%) of multi drug resistant isolates in UBTH and NAUTH respectively. Cefixime had the highest resistance at 94.0% from both locations followed by augmentin and cefuroxime which resistance ranged from 68.0% to 94.0% and 68.0% to 79.0% respectively. The least resistance were observed in erythromycin and oxacillin with a range of 0.0% to 17.0%. There was high multidrug resistance to isolates obtained from both locations. Plasmid DNA profiling was carried out on all multi drug resistant isolates on agarose gel and photographed using ultraviolet transilluminator. The isolates were positive for plasmid DNA suggesting that plasmids were established in drug resistant P. aeruginosa associated with surgical infections in the study area

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